Semiquantitative scoring of histological spironolactone effects. Thirteen renal histopathological parameters were blindly scored from 0 (unaffected) to 3 (severely affected): (a) cellular crescent score, (b) mesangial cellularity score, (c) activity score, and (d) chronicity score. At 36 weeks, there were significant differences in cellular crescent formation between treated and control mice (panel a; *P = 0.05) and better scores in treated mice in most other parameters (representative in panel b). A combined score revealed treated mice to have a better activity index (panel c; P = 0.10). Chronicity indices were similar compared with control mice (panel D). Data are presented as the percentage of mice in each treatment group that had the indicated score; each treatment group included seven or eight mice by the 36-week time point.
Monrad et al. Arthritis Research & Therapy 2008 10:R5 doi:10.1186/ar2353