Conventional RT-PCR for the house-keeping gene GAPDH, semaphorin 3A, 3F and their receptors. This is a representative photograph following agarose gel electrophoresis of products from reverse transcription polymerase chain reaction for GAPDH, sema3A, sema3F, NRP-1, NRP-2, PLXA1, PLXA2, PLXA3, PLXA4 and PGP9.5. cDNA samples consisted of 14 healthy (non-degenerate) and 14 surgical (degenerate)intervertebral disc samples, with seven annulus fibrosus and seven nucleus pulposus in each. A 100 bp Hyperladder IV molecular weight marker (Mw) was included to allow estimation of correct band molecular weights. * represents samples assumed to be NP due to the nature of surgical intervention and sample collection technique.
Tolofari et al. Arthritis Research & Therapy 2010 12:R1 doi:10.1186/ar2898