Quantitative real-time PCR for typical chondrocyte marker genes in bovine AC, AF and NP cells. Relative gene expression for the chondrocyte marker genes (type II collagen (COL2A1), aggrecan (ACAN) and versican (VCAN)) was normalised to the housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and plotted on a log scale. * statistical significance between nucleus pulposus (NP) and annulus fibrosus (AF) cells and nucleus pulposus (NP) and AC cells (P < 0.05). † statistical significance between AF cells and AC cells (P < 0.05).
Minogue et al. Arthritis Research & Therapy 2010 12:R22 doi:10.1186/ar2929