Patients with SLE have an increased frequency of Th17 cells but not Th1 cells. PBMCs from patients with SLE (n = 25) and healthy control subjects (HC, n = 26) were stimulated for 4 hours ex vivo with PMA and ionomycin or PBS (control) in the presence of Golgiplug. The frequency of CD4+ T cells producing IL-17 and/or IFN-γ was measured by using flow cytometry. (a) Representative dot plots showing CD4+ T cells producing IL-17 and/or IFN-γ. (b) The frequency (% of CD4+IL-17+ T cells, CD4+IL-17+IFN-γ+ T cells and CD4+IFN-γ+ T cells in patients with SLE and in healthy control subject (HC). Numbers in dot plots indicate the frequency of cells for each quadrant. Bars show the means.
Shah et al. Arthritis Research & Therapy 2010 12:R53 doi:10.1186/ar2964