Changes in gene expression during passaging. (a) The changes in gene expression increase substantially during passaging of the cells in all fibroblast cultures. In addition, the variations and differences between expression pattern in the different rheumatoid arthritis synovial fibroblasts (RASF) cultures increase also in later passages (% changes of gene expression ± standard error of the mean). The regression line shows a clear increment in the course of the culture of the cells (red). (b) Changes in gene expression comparing higher passages instead of passage 1. (c) Verification of CD82 and c-myc regulation using real-time PCR. Passaging was performed in two parallel cultures for each fibroblast population. Real-time PCR was performed threefold (six measurements for each population, respectively).
Neumann et al. Arthritis Research & Therapy 2010 12:R83 doi:10.1186/ar3010