Ratios of Fox-P3/PBMCs, CCR4+CCR6+ Th/PBMCs, Treg/PBMCs and CCR4+CCR6+ Th/Treg. Peripheral blood mononuclear cells (PBMCs) were collected to be assayed by flow cytometry for the CCR4+CCR6+ T-helper (Th) cells, regulatory T (Treg) cells and forkhead box P3 (Fox-P3)-positive cells after co-culture with or without (3 day-control) ankylosing spondylitis (AS)-bone marrow-derived mensenchymal stem cells (BMSCs) (3 day-ASp), HLA-B27-negative healthy donors (HD1)-BMSCs (3 day-HD1) or HLA-B27-positive healthy donors (HD2)-BMSCs (3 day-HD2) for 72 hours. Statistical multiple bar graphs show that AS-BMSCs induced the ratio of CCR4+CCR6+ Th/Treg imbalance via reducing Treg/PBMCs but increasing CCR4+CCR6+ Th/PBMCs; it also produced a significant reduction of Fox-P3-positive cells in PBMCs (*P < 0.001, Student's t test for independent data).
Wu et al. Arthritis Research & Therapy 2011 13:R29 doi:10.1186/ar3257