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Ossification process involving the human thoracic ligamentum flavum: role of transcription factors

Kenzo Uchida1, Takafumi Yayama1*, Hong-Xin Cai12, Hideaki Nakajima1, Daisuke Sugita1, Alexander Rodríguez Guerrero13, Shigeru Kobayashi1, Ai Yoshida1, Ke-Bing Chen14 and Hisatoshi Baba1

Author Affiliations

1 Department of Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, Fukui University, Eiheiji, Fukui 910-1193, Japan

2 Department of Orthopedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, People's Republic of China

3 Servicio de Neurocirugia, Hospital Nacional Rosales, Universidad de El Salvador, San Salvador 106-8000, El Salvador

4 Department of Spinal Surgery, The First Affiliated Hospital, Sun Yat-Sen University School of Medicine, Guangzhou 510080, People's Republic of China

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Arthritis Research & Therapy 2011, 13:R144  doi:10.1186/ar3458

Published: 13 September 2011

Additional files

Additional file 1:

Topographic analysis of the expression of transcriptional factors. The table shows the summary of immunohistochemical localization of Sox9, Runx2, Msx2, Osterix, Dlx5, and AP-1 in all 31 cases. The tabulated data represent the distribution of immunopositive areas in the ossification front, based on semiquantitative analysis conducted according to the method described by Kokubo et al. [22] and Song et al. [35].

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Additional file 2:

Relation between CT subtype and immunopositivity to each transcriptional factor. The results indicate that the expression of transcriptional factors varied according to the size of the ossified plaque subtype. Immunopositivity for Sox9, Runx2, and Msx2 tended to be more common in the fused and tuberous subtypes (near 100%) than in the lateral and extended subtypes (< 50%). Expression of Osterix, Dlx5, and AP-1 was also high in the fused and tuberous subtypes.

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