Additional file 1.

Figure S1. Evaluation of hRasGRP4 transcripts in CD14+ peripheral blood monocytes and in vitro cultured macrophages or osteoslasts. A. hRasGRP4 transcripts in CD14+ peripheral blood monocytes and in-vitro cultured macrophages were evaluated using real-time qPCR. The level of hRasGRP4 transcripts against GAPDH in CD14+ cells at the first experiment was defined as 1. B. hRasGRP4 transcripts in in-vitro cultured osteoclasts were measured by real-time qPCR (left panel). Expression of cathepsin K (CTSK) was measured to confirm the development of osteoclasts (right panel). In these panels, amount of target gene transcripts against GAPDH transcripts in PBMC at one experiment were defined as 1. All experiments were done in a triplicate manner and error bars indicate standard errors. OC, osteoclasts; PBMC, peripheral blood mononuclear cells; RQ, relative quantification.

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Hashimoto et al. Arthritis Research & Therapy 2011 13:R154   doi:10.1186/ar3470