Immunohistochemical and immunoblot analyses. Immunohistochemistry, western blotting, and quantification of type II collagen, type I collagen, and matrix metalloproteinase (MMP)-13 expressions at week 5 after injection. (A) Immunohistochemical analysis for type II collagen. Bar = 50 μm. (B) Western blot analysis of type II collagen, type I collagen, and MMP-13 (pooled samples in each group, n = 5). (C) Quantitative analysis of the expressions of type II collagen, type I collagen, and MMP-13 relative to the intensity of β-actin. Data are mean ± standard deviation of five guinea pigs in each group. *P < 0.05. HA, hyaluronic acid; MSC, mesenchymal stem cell.
Sato et al. Arthritis Research & Therapy 2012 14:R31 doi:10.1186/ar3735