MKK7 ASO reduces kinase phosphorylation in K/BxN serum transfer arthritis. Ankle joints were collected at Day 10 after treatment with MKK7 ASO and homogenized as described. Western blot analysis was used to determine relative phosphorylation of MKK4, JNK, and c-Jun. (A) Representative experiment showing total and phosphorylated proteins. (B) Quantitative analysis of Western blot analysis after normalizing results to GAPDH. Values = Mean ± SE, n = 3 for each group, *P < 0.05 vs. control ASO.
Lee et al. Arthritis Research & Therapy 2012 14:R38 doi:10.1186/ar3750