Additional file 1.

Effect of cycloheximide on TNF-α induced GLS mRNA production in FLSs. Confluent FLSs in 6-well plate were incubated in the presence or absence of 10-100 (μg/ml cycloheximide for 30 min, followed by further incubation with TNF-α (1 ng/ml) for 24 h. The GLS mRNA levels are expressed as a RT-PCR product ratio (GLS/β-actin). Results are presented as mean ± SEM of four determinations. Statistical significance compared with controls was calculated using the Mann-Whitney U-test: compared to controls * P < 0.01; compared to samples with TNF-α alone † P < 0.01.

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Ikuta et al. Arthritis Research & Therapy 2012 14:R87   doi:10.1186/ar3811