Effect of interleukin-6 (IL-6) on methotrexate (MTX)-induced anti-proliferative effect. Synoviocytes from arthritic mice were cultured for 24 h with methotrexate (0, 10, 100, 1,000 nM) with medium alone or in the presence of IL-6 (100 ng/mL) + soluble IL-6 receptor (sIL-6R) (100 ng/mL). After culturing, cell proliferation was measured by uptake of bromodeoxyuridine. Statistical significance was analyzed by the unpaired t-test (*P < 0.05). Each column and vertical line indicates the mean and standard deviation of quadruplicate cultures.
Hashizume et al. Arthritis Research & Therapy 2012 14:R96 doi:10.1186/ar3821