SAA induces the transcription of pro-IL-1β in human synovial fibroblasts. A. Synovial fibroblasts were incubated with SAA (2 μg/ml) for the indicated periods. The cells were harvested and analyzed for pro-IL-1β mRNA levels by real-time PCR. B. Synovial fibroblasts were incubated with the indicated concentrations of SAA for 6 h. The cells were harvested and analyzed for IL-1β and GAPDH mRNA levels by real-time PCR. Values represent the mean ± SD of three independent experiments.*P <0.001 compared to SAA-untreated synovial fibroblasts.
Migita et al. Arthritis Research & Therapy 2012 14:R119 doi:10.1186/ar3849