SAA induces the secretion of cathepsin B from synovial fibroblasts. Synovial fibroblasts were untreated or pretreated with SAA (2 μg/ml) for 12 h and subsequently stimulated with of MSU (200 μg/ml) for 24 h. Supernatants were analyzed by immunoblot analysis for presence of cathepsin B. Three experiments were performed using different synovial fibroblasts and a representative result is shown.
Migita et al. Arthritis Research & Therapy 2012 14:R119 doi:10.1186/ar3849