TLR7-/- mice have decreased cytokine expression in the paw tissue. RNA was isolated from whole affected paws of control C57Bl/6 wild-type (WT) (n = 4 or 5) or TLR7-/- (n = 3 or 4) mice. Real-time polymerase chain reaction was performed to measure levels of tumor necrosis factor-alpha (TNFα), interleukin-1-beta (IL-1β), IL-6, IL-17, and interferon-gamma (IFNγ) using ABI primers. *P < 0.05. TLR, Toll-like receptor.
Alzabin et al. Arthritis Research & Therapy 2012 14:R142 doi:10.1186/ar3875