Establishment of IL-17-producing Th17 cells from uveitis patients with Behçet's disease. (A) Purified CD4+ T cells from a Behçet's disease (BD) patient or a sarcoidosis (SAR) patient (black bars) with active uveitis or a healthy donor (HD, open bars) in the presence of anti-CD3/CD28 antibodies, anti-IFN-γ antibody, anti-IL-4 antibody, rIL-1β, rIL-6, rIL-23, and rTNF-α. For ELISA analysis, supernatants of polarized Th17 cell lines or control CD4+ T cell lines in the presence of only anti-CD3/CD28 antibodies were harvested. The graph indicates the amount of IL-17 determined by ELISA (pg/ml). (B) For flow cytometric analysis, harvested Th17 cell lines from BD or HD were stained with anti-IL-17 abs and anti-CD4 Abs after permeabilization. The numbers in the histograms indicate the percentages of cells that were double-positive for IL-17/CD4. Abs, antibodies.
Sugita et al. Arthritis Research & Therapy 2012 14:R99 doi:10.1186/ar3824