TG2 knockdown reduces collagen degradation in CIA rats. Representative images (10× magnification) of immunostaining of (A) TG2, (B) ε-(γ-glutamyl)-lysine bonds, and (C) degraded collagen in the synovial membranes from control rats (PBS; n = 5), CIA rats injected with control (CIA+Ctl-sh; n = 6), or TG2 (CIA+TG2-sh; n = 5) shRNA-expressing lentivirus. Associated graphs show relative labeling intensities for these experimental groups and CIA control (n = 4) rats, calculated from three random fields for each tissue section. Values are expressed as the mean ± SEM (***P < 0.001).
Lauzier et al. Arthritis Research & Therapy 2012 14:R159 doi:10.1186/ar3899