Measuring bio-mineralization in Saos-2 cells cultured with OM and acetazolamide (AZ). The Saos-2 cells were incubated with normal medium, osteogenic medium (OM), and OM supplemented with 100 μM of AZ. A. The cell cultures were stained with AR-S to detect calcium nodules and photographed at the original magnification and 100X magnification. B. The cell cultures were stained with cetylpyridinium chloride and quantified by measuring its absorbance at 562 nm. This experiment was repeated three times. ** = P < 0.01, *** = P < 0.001.
Chang et al. Arthritis Research & Therapy 2012 14:R176 doi:10.1186/ar3929