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Human monocytes and macrophages differ in their mechanisms of adaptation to hypoxia

Monique Fangradt12, Martin Hahne123*, Timo Gaber124, Cindy Strehl12, Roman Rauch1, Paula Hoff12, Max Löhning12, Gerd-Rüdiger Burmester1 and Frank Buttgereit124

  • * Corresponding author: Martin Hahne

  • † Equal contributors

Author Affiliations

1 Department of Rheumatology and Clinical Immunology, Charité University Hospital, Charitéplatz 1, Berlin, 10117 Germany

2 German Rheumatism Research Center (DRFZ), Charitéplatz 1, Berlin, 10117 Germany

3 Berlin-Brandenburg School for Regenerative Therapies (BSRT), Foehrer Straße 15, Berlin, 13353 Germany

4 Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Foehrer Straße 15, Berlin, 13353 Germany

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Arthritis Research & Therapy 2012, 14:R181  doi:10.1186/ar4011

Published: 7 August 2012

Additional files

Additional file 1:

Figure S1 A-C. Myeloid cell lines (THP-1, HL-60, and U937) express HIF-1α in the cell nucleus. THP-1 cells (A), HL-60 cells (B) and U937 cells (C) express HIF-1α in the nucleus under normoxia and hypoxia, with or without PMA stimulation (5 h). THP-1 and U937 show similar expression of HIF-1α under normoxia and hypoxia (A,C), whereas HL-60 cells demonstrate increased expression of hypoxia-inducible factor (HIF)-1α under hypoxia (B). All cell lines show a higher expression of HIF-1α in the presence of PMA (A-C). Detection of HIF-1α, Lamin B and β-actin in nuclear (NF+) and cytosolic (NF-) cell fractions of myeloid cell lines using immunoblot. (A-C) Protein lysates were acquired after incubation for 5 h under hypoxia and under normoxia as indicated.

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