Adiponectin receptor expression in scleroderma. A. Total RNA was isolated from confluent fibroblasts explanted from scleroderma patient (n = 4) and healthy controls (n = 4), and subjected to real-time qPCR. The results represent the mean ± SEM of triplicate determinations. B. AdipoR1/2 mRNA expression was interrogated in publicly available genome-wide expression scleroderma skin biopsy microarray datasets (GEO accession number: GSE9285) . Fold-change in mRNA levels was normalized with average expression levels of the samples in the entire cohort. Box plots indicate the range of lower and upper quartiles. * P <0.05. SEM, standard error of the mean.
Fang et al. Arthritis Research & Therapy 2012 14:R229 doi:10.1186/ar4070