OA cartilage explant culture with inhibition of synovial fluid IL-6 or addition of exogenous IL-6. (a, c) GAG content and GAG release in OA cartilage explants from three donors cultured either in medium only, medium supplemented with 25% OA synovial fluid (SF) or medium with 25% OA synovial fluid in which IL-6 is blocked with an activity-inhibiting antibody (mean ± SD in mg/g); P = 0.06. (b, d) GAG content and GAG release in OA cartilage explants from eight donors cultured in the presence or absence of rhIL-6 (50 ng/mL) with rhIL-6Rα (200 ng/mL).
Tsuchida et al. Arthritis Research & Therapy 2012 14:R262 doi:10.1186/ar4107