Email updates

Keep up to date with the latest news and content from Arthritis Research & Therapy and BioMed Central.

This article is part of the supplement: Proceedings of the 8th Global Arthritis Research Network (GARN) Meeting and 1st Bio-Rheumatology International Congress (BRIC)

Open Badges Poster presentation

LC-MS/MS-based shotgun proteomics identified the targets of arthritis-related microRNA

Riho Kurata12*, Tomo Yonezawa1, Hideki Nakajima3, Shyuji Takada1 and Hiroshi Asahara145

Author Affiliations

1 Department of Systems BioMedicine, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo 157-8535, Japan

2 Department of Molecular Life Sciences, Basic Medical Science and Molecular Medicine, Tokai University School of Medicine, Isehara, Kanagawa, Japan

3 Department of Pediatric Hematology and Oncology Research, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo 157-8535, Japan

4 Department of Systems BioMedicine, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 113-8510, Japan

5 Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Saitama 332-0012, Japan

For all author emails, please log on.

Arthritis Research & Therapy 2012, 14(Suppl 1):P36  doi:10.1186/ar3637

The electronic version of this article is the complete one and can be found online at:

Published:9 February 2012

© 2012 Kurata et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Poster presentation

microRNAs (miRNAs), which are class of post-transcriptional regulators such as short 19 to 23-nucleotide non-coding RNAs, complementarily bind seed sequences in the 3'-untranslational region of multiple target mRNAs, resulting in their suppression of translation or degradation [1]. In the former case, since the mRNA expression of the targets does not any change, transcriptomics approach, such as expression array, cannot identify the targets.

Recent studies shed light on the fine-tuning mechanism of miRNAs in myriad biological processes including development [2], tumorigenesis [3] and inflammation [4]. We have identified enhancement of mir-146a expression in rheumatoid arthritis synoviocyte and macrophages [5], whilst suppression of them in osteoarthritis [6]. Another group also have identified the enhancement of mir-146a and mir-155 in response to bacterial pathogen such as lipopolysaccaride [7]. Recently, mice lacking of mir-155 are resistant to collagen-induced arthritis (CIA) [8], whilst administration of mir-146a complexed with aterocollagen into joint attenuates pathological condition of CIA [9]. These results indicate that mir-146a and mir-155 plays an important role for developing arthritis and inflammation. However, the targets of both two miRNAs and their molecular mechanisms are not still fully identified.

In this study, in order to identify the targets of them in translational level, we established gain of function models using adenovirus- and CMV promoter-mediated overexpression in several culture models and performed liquid chromatography-tandem mass spectrometry-based shotgun proteomics in these models.


The authors sincerely thank Dr. Yanagiya R for helpful advice on preparation of adenovirus, and Dr. Inoue A for the gift of CMV vector for mir-146a.


  1. Lee RC, Feinbaum RL, Ambros V: The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.

    Cell 1993, 75:843-854. PubMed Abstract | Publisher Full Text OpenURL

  2. Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Horvitz HR, Ruvkun G: The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans.

    Nature 2005, 403:901-906. OpenURL

  3. Johnson SM, Grosshans H, Shingara J, Byrom M, Jarvis R, Cheng A, Labourier E, Reinert KL, Brown D, Slack FJ: RAS is regulated by the let-7 microRNA family.

    Cell 2005, 120:635-647. PubMed Abstract | Publisher Full Text OpenURL

  4. Lu LF, Boldin MP, Chaudhry A, Lin LL, Taganov KD, Hanada T, Yoshimura A, Baltimore D, Rudensky AY: Function of miR-146a in controlling Treg cell-mediated regulation of Th1 responses.

    Cell 2010, 142:914-929. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  5. Nakasa T, Miyaki S, Okubo A, Hashimoto M, Nishida K, Ochi M, Asahara H: Expression of microRNA-146 in rheumatoid arthritis synovial tissue.

    Arthritis Rheum 2008, 58:1284-1292. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  6. Yamasaki K, Nakasa T, Miyaki S, Ishikawa M, Deie M, Adachi N, Yasunaga Y, Asahara H, Ochi M: Expression of MicroRNA-146a in osteoarthritis cartilage.

    Arthritis Rheum 2009, 60:1035-1041. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  7. Taganov KD, Boldin MP, Chang KJ, Baltimore D: NF-kappaB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses.

    Proc Natl Acad Sci USA 2006, 103:12481-12486. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  8. Kurowska-Stolarska M, Alivernini S, Ballantine LE, Asquith DL, Millar NL, Gilchrist DS, Reilly J, Ierna M, Fraser AR, Stolarski B, McSharry C, Hueber AJ, Baxter D, Hunter J, Gay S, Liew FY, McInnes IB: MicroRNA-155 as a proinflammatory regulator in clinical and experimental arthritis.

    Proc Natl Acad Sci USA 2011, 108:11193-11198. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  9. Nakasa T, Shibuya H, Nagata Y, Niimoto T, Ochi M: The inhibitory effect of microRNA-146a expression on bone destruction in collagen-induced arthritis.

    Arthritis Rheum 2011, 63:1582-1590. PubMed Abstract | Publisher Full Text OpenURL