Real time PCR analysis for Aggrecan, Collagen type II, and Collagen type I-mRNA-expression. mRNA expression for Aggrecan (A), Collagen type II (B), and Collagen type I (C) was assessed in fresh cartilage, in resident chondrocytes from the matrix of cultured (+/-TGF-β1) cartilage, as well as in cells emigrated from cultured cartilage (+/-TGF-β1) onto the cartilage surface or the BNC matrix. Values are expressed as means ± SEM. BNC, bacterial nana-cellulose; SEM, standard error of the mean; TGF-β1, transforming growth factor-β1.
Pretzel et al. Arthritis Research & Therapy 2013 15:R59 doi:10.1186/ar4231