Localization of Sox9, SZP and GDF-5. Sections of the tibial articular cartilage of 12-week-old normal Mig-6-flox (A, C, E) and Mig-6-flox;Prx1Cre conditional knockout (Mig-6-cko) knees (B, D, F) immunostained with antibodies against Sox9 (A, B); superficial zone protein (SZP, C, D); and growth and differentiation factor-5 (GDF5, E, F). In normal articular cartilage, immunostaining is localized to cells mainly in the superficial zone (arrows in A, C, E) while in Mig-6-cko articular cartilage, immunostaining is dramatically increased and positive cells are located not only in the superficial zone but also in deeper zones (arrows in B, D, F). Scale Bar = 50 μm (A-D); 25 μM (E, F).
Shepard et al. Arthritis Research & Therapy 2013 15:R60 doi:10.1186/ar4233