Additional Figure 2.

Mig6 expression, EGFR activation and proliferation in menisci and/or synovium. Sections of the menisci of normal six-week-old Mig-6-flox knees (A, C, E) and Mig-6-flox;Prx1Cre conditional knockout (Mig-6-cko) knees (B, D, F) immunostained with antibodies against Mig-6 (A, B); phosphorylated EGFR (p-EGFR, C, D); or Ki67 (E, F). (A, B) Endogenous localization of Mig-6 protein in the superficial region of the meniscus (arrowheads in A) which is absent in the knockout animal (B). (C-F) EGFR signaling and proliferation are occurring within the superficial region of the meniscus (arrowheads) of the normal Mig-6-flox knee (C, E), and EGFR signaling and proliferation is enhanced and extends further into the meniscus in the Mig-6-cko knee (compare D, F to C, E). In addition, immunostaining for pEGFR is also present in the thickened Mig-6-cko synovial tissue (arrow in D). Scale Bar = 100 μm.

Format: TIFF Size: 4.8MB Download file

Shepard et al. Arthritis Research & Therapy 2013 15:R60   doi:10.1186/ar4233