Expression of transcription factors BCL-6 and FoxP3. (A) Representative FACS plots of BCL-6 versus FoxP3 expression in circulating CD4+ T-cells from an antineutrophil cytoplasmic antibody (ANCA)-negative (right plot) and an ANCA-positive (middle plot) patient with granulomatosis with polyangiitis (GPA), and an age- and sex-matched healthy control (HC) (left plot). Values in each gate represent the percentages of positive cells. (B) Relative mRNA expression of BCL-6 in leukocytes from ANCA-positive (n = 10) and ANCA-negative (n = 6) GPA patients, and age- and sex-matched HCs (n = 11) was analyzed by real-time reverse transcription-PCR normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (C) Percentage of BCL-6+FoxP3- cells in circulating CD4+ T-cells was determined in ANCA-positive (n = 11) and ANCA-negative (n = 10) GPA patients, and age- and sex-matched HCs (n = 10). Bars represent the mean values ± SD. P-values were calculated using the nonparametric Mann-Whitney U-test. *P < 0.05; **P < 0.005.
Abdulahad et al. Arthritis Research & Therapy 2013 15:R70 doi:10.1186/ar4247