Additional File 2.
figure showing ASC viability with single and combined adenoviral transduction. Monolayers were transduced with increasing doses of Ad.GFP, Ad.IGF-1, Ad.TGF-β1, Ad.FGF-2 and Ad.SOX9 (A) alone and (B) in combination. At 10 days, cell viability was measured with the Alamar Blue assay; the optical density OD570 to 600 nm values of untransduced cells were set as 100%. Data expressed as mean standard error of triplicate experiments. (C) At 72 hours, GFP-positive cells were counted in three fields under light and fluorescence microscopy. Results are presented as the mean percentage of fluorescent cells per field at each viral dose. (D) Representative fluorescence of ASCs transduced with 1, 10, 100, and 1,000 MOIs of Ad.GFP, as indicated.
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Garza-Veloz et al. Arthritis Research & Therapy 2013 15:R80 doi:10.1186/ar4260