Open Access Open Badges Research article

Hypercholesterolemia boosts joint destruction in chronic arthritis. An experimental model aggravated by foam macrophage infiltration

I Prieto-Potín, JA Roman-Blas, MJ Martínez-Calatrava, R Gómez, R Largo and Gabriel Herrero-Beaumont*

Author Affiliations

Bone and Joint Research Unit, Service of Rheumatology, IIS Fundación Jiménez Díaz, Universidad Autónoma, Av. Reyes Católicos 2, 28048, Madrid, Spain

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Arthritis Research & Therapy 2013, 15:R81  doi:10.1186/ar4261

Published: 13 August 2013

Additional files

Additional file 1:

High fat diet (HFD) and antigen induced arthritis (AIA) interventions increase TNF-α (A) and MCP-1 (B) gene expression in the synovium. Gene expression was examined by real time PCR and results are expressed as fold induction. Bars show the mean and SEM (n = 7 to 9 rabbits per group).

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Additional file 2:

RANKL and OPG protein expression. A, C and E, Densitometric analysis of receptor activator nuclear kappa B ligand (RANKL) and osteoprotegerin (OPG) protein expression in cartilage (A), subchondral bone (C) and synovium (E). Bars show the mean and SEM (n = 7 to 9 rabbits per group). B, D and F, Representative Western blot images for RANKL and OPG detection in cartilage (B), subchondral bone (C) and synovium (F). EZ Blue-stained gels used as protein loading controls are also shown for healthy rabbits, high fat diet (HFD) rabbits, chronic antigen induced arthritis (AIA) rabbits and HFD + AIA rabbits.

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