Seven IRF5 polymorphisms showing differential electrophoretic mobility shift assay results. Seven of the twenty-six polymorphisms analyzed by electrophoretic mobility shift assay (EMSA) showed differential patterns of delayed migration with their two alleles. (A) rs729302. (B) rs12706860. (C) rs13245639. (D) rs3778754. (E) rs3807307. (F) rs17424179. (G) rs11269962. They were analyzed with WIL2 NS cell nuclear extracts, and the specificity of the bands was checked by competition with unlabeled oligonucleotides in 200-fold excess. Each difference was established in three independent EMSAs with concordant results. Sequences of the probes are given in Additional file 2: Table S4.
Alonso-Perez et al. Arthritis Research & Therapy 2013 15:R82 doi:10.1186/ar4262