Flow cytometry of CD148 in peripheral blood and synovial fluid cells of rheumatoid arthritis patients. Cells were isolated either from peripheral blood or synovial fluid from RA patients or control patients as described in Materials and methods and subjected to flow cytometric analysis for CD148 and immune cell markers. (A) Flow cytometry of CD148 in PMNs from blood or synovial fluid of RA patient. (B) Flow cytometry of CD148 expression in CD8-positive or CD4-positive T cells (upper panels) from normal, RA patient blood or synovial fluid. (C) Quantitation of CD148 in CD8/CD45RO or CD4/CD45RO double positives. (D) Quantitation of CD148 expression in CD14-positive macrophages. In panels A, B and D clear areas under the curve are isotype control, grey shaded areas are anti-CD148, and filled black shading shows the expression of CD148 above isotype control. MFI, mean fluorescence intensity; PMN, polymorphonuclear neutrophils; RA, rheumatoid arthritis; n = 3; error bars depict SEM.
Dave et al. Arthritis Research & Therapy 2013 15:R108 doi:10.1186/ar4288