Effects of BMS-345541 or/and wortmannin on the LPS-induced proinflammatory and apoptotic signaling in human chondrocytes. Primary human chondrocytes in monolayer cultures were either left untreated or stimulated with 100 ng/ml lipopolysaccharides (LPS), prestimulated with 5 mM BMS-345541, with 20 nM wortmannin or a combination of both inhibitors, BMS-345541 and wortmannin (5 mM and 20 nM) for 12 h and then treated with 100 ng/ml LPS for the indicated times. Whole-cell extracts were fractionated (500 ng protein per lane) on SDS-PAGE and examined by western blot analysis using anti-matrix metalloproteinase (MMP)-9 (A), -MMP-13 (B), -cyclooxygenase-2 (Cox-2) (C) and anti-cleaved caspase-3 (D). The results shown are representative of three independent experiments. Housekeeping protein β-actin served as a loading control.
Lorenz et al. Arthritis Research & Therapy 2013 15:R111 doi:10.1186/ar4291