Additional file 3.

Figure S3. Gating strategy for the analysis of the suppressive capacity of lymphocytes primed with various tolDC populations. Naïve responder cells were labeled with CFSE and tolDC-primed NAC were labeled with CellTrace Violet. This allowed exclusive gating on CellTrace Violet-negative cells and further analysis of proliferation of CFSE-labeled responder cells. The top left dot plot depicts cells in a live gate based on forward scatter and side scatter, the top right histogram depicts gating on CellTrace Violet-negative cells. The bottom histogram shows proliferation of CFSE-labeled CellTrace Violet-negative responder cells after co-culture with differently primed NAC. MFI values of proliferated responder cells and tolDC populations that were used to stimulate effector cells are shown in the histograms legend. MFI, median fluorescence intensity; NAC, nonadherent cells; tolDC, tolerogenic dendritic cells.

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Volchenkov et al. Arthritis Research & Therapy 2013 15:R114   doi:10.1186/ar4294