Immunostaining for α-SMA (arrows, myofibroblasts nuclei) in cutaneous samples. Representative tissue sample from: (A) Sham animal; (B) HOCl mice; (C) HOCl + PTU animal (Original magnification, ×40). The arrows show strong diffuse staining of myofibroblasts nuclei (dark brown staining); (D) Number of myofibroblasts from the three experimental groups (HOCl + PTU group, n = 10; HOCl group, n = 10; Sham, n = 5). The increase of myofibroblast population in the skin of HOCl mice is prevented by propylthiouracil administration. Values are expressed as the mean and SD. *P < 0.001 versus Sham; #P < 0.001 versus HOCl.
Bagnato et al. Arthritis Research & Therapy 2013 15:R120 doi:10.1186/ar4300