Additional file 2: Figure S1.

Showing evaluation of cell viability after introduction of siRNA, an assay using MTT. siRNA for α5 (ITGA5) or β1 integrin gene (ITGB1) was introduced into primary cultured chondrocytes, and the cells were subjected to MTT assay 3 days after the introduction. A commercially available kit (Cell Proliferation Kit I; Roche Diagnostics) was used for the assay. Results are shown by relative ratios against cells given control siRNA. Three bars for each integrin gene indicate results of three siRNAs used for the gene. Results are mean ± standard error of the mean of three independent experiments, each in triplicate. GADPH, glyceraldehyde 3-phosphate dehydrogenase.

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Tanaka et al. Arthritis Research & Therapy 2013 15:R127   doi:10.1186/ar4307