Additional file 4: Figure S3.

Showing that primary cultured articular chondrocytes were maintained in monolayers, and expression of α5 (ITGA5) (a) and β1 integrins (ITGB1) (b) was determined respectively 2 days (D2) and 7 days after plating (D7) by quantitative PCR. Expression levels at 7 days are shown by relative ratios against those at 2 days. (c) Expression of α5β1 integrin in monolayer-cultured chondrocytes was evaluated by flow cytometry at 2 days (shaded areas with fine lines) and 7 days (open areas with bold lines) after plating using a mAb for that integrin (10 μg/ml, HA5; Millipore). Representative results of five independent experiments are shown. (d) In flow cytometric analysis, fluorescence intensity for α5β1 integrin at 7 days (D7) was shown by relative ratio against that at 2 days (D2). (a), (b), (d) Results are mean ± standard error of the mean of five independent experiments. GADPH, glyceraldehyde 3-phosphate dehydrogenase.

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Tanaka et al. Arthritis Research & Therapy 2013 15:R127   doi:10.1186/ar4307