Analysis of cytokine levels in mice with full collagen-induced arthritis. At the end of the therapy 13 different cytokine concentrations were measured in plasma using multiplex bead-based flow cytometry. Data points of cytokine concentrations above detection level are represented in a scatter plot with the mean ± standard error of the mean (n = 7). Standard curves defined with positive control samples (Additional file 5) were used to generate a level of quantification (LOQ; dotted red line). Most of the measured concentrations were below the LOQ, but a significant increase in the level of IL-27 was observed for F8-huIL10 and the combination versus saline (*P = 0.0017 and P = 0.038, respectively). IFN, interferon; IL, interleukin; TNF, tumor necrosis factor.
Doll et al. Arthritis Research & Therapy 2013 15:R138 doi:10.1186/ar4319