Additional file 2.
Complete sequence of F8-muIL10. The sequence for murine IL10 (amino acids 19 to 178) was appended at the C-terminus of the F8 antibody in diabody format, with a five-amino-acid linker between variable heavy chain (VH) and variable light chain (VL) and a 15-amino-acid linker ((SSSSG)3) between the antibody and IL-10. At the N-terminus a signal sequence (SS) was added. By NheI and NotI double digest the insert was included into the mammalian cell-expression vector pcDNA3.1(+).
Format: PDF Size: 319KB Download file
This file can be viewed with: Adobe Acrobat Reader
Doll et al. Arthritis Research & Therapy 2013 15:R138 doi:10.1186/ar4319