Additional file 8.
Anti type-II collagen antibodies. Titers of bovine type-II collagen-specific total IgG, IgG1 and IgG2a antibodies were determined using standard ELISA techniques. Briefly, 96-well plates were coated with 5 μg/ml bovine collagen II solution (Chondrex, Inc., Redmond, WA, USA) overnight at 4°C. Plasma samples diluted 1:800 were incubated for 1 hour and detected with horseradish peroxidase conjugated anti-mouse IgG, IgG1 and IgG2a antibodies (Santa Cruz, Heidelberg, Germany). Healthy*, mice were immunized but did not show any signs of inflammation.
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Doll et al. Arthritis Research & Therapy 2013 15:R138 doi:10.1186/ar4319