Additional file 3: Figure S2.
Effect of IFN-α on intracellular cytokine expression in mock-stimulated macrophages and mock- and anti-CD3-stimulated T cells from lymph nodes during AIA. Draining lymph node cells were collected from mice at days 0, 14, 21, and 28 during the course of AIA and restimulated with complete media (mock) or anti-CD3 (1 μg/ml) for 24 hours. Brefeldin A (5 μg/ml) and monensin (1 μg/ml) were added 5 hours before FACS analysis. (A-F) Percentage IFN-γ-, IL-17A-, and TGF-β-expressing lymph node cells of gated CD4+ (A-C) and F4/80+ cells (D-F). The data shown are mean values ± SEM of pooled data from two independent experiments (n ≥ 5) with similar results (*P < 0.05). Blue line, mice immunized in the presence of IFN-α; black line, control.
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Chalise et al. Arthritis Research & Therapy 2013 15:R143 doi:10.1186/ar4326