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Glycophenotyping of osteoarthritic cartilage and chondrocytes by RT-qPCR, mass spectrometry, histochemistry with plant/human lectins and lectin localization with a glycoprotein

Stefan Toegel1*, Daniela Bieder1, Sabine André2, Friedrich Altmann3, Sonja M Walzer1, Herbert Kaltner2, Jochen G Hofstaetter14, Reinhard Windhager1 and Hans-Joachim Gabius2

Author Affiliations

1 Karl Chiari Lab for Orthopaedic Biology, Department of Orthopaedics, Medical University of Vienna, Waehringer 18-20 1090 Vienna, Austria

2 Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Munich, Germany

3 Department of Chemistry, University of Natural Resources and Life Sciences, Vienna, Austria

4 2nd Department, Orthopaedic Hospital Vienna-Speising, Vienna, Austria

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Arthritis Research & Therapy 2013, 15:R147  doi:10.1186/ar4330

Published: 4 October 2013

Additional files

Additional file 1: Table S1:

Characteristics of clinical specimens. OA cartilage was obtained from OA patients (age range 54 to 80 years) between September 2010 and April 2012 according to the protocol given in the Methods section.

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Additional file 2: Table S2:

MIQE checklist of RT-qPCR assays.

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Additional file 3: Table S3:

Localization of lectin-reactive glycans in OA cartilage.

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