Summary of the ex vivo test conditions and types of analysis. Chondrocyte/agarose constructs were cultured ex vivo under free-swelling conditions (1, 5 and 21%) or subjected to dynamic compression (15%, 1 Hz) at 5 and 21% oxygen tension. At the end of the experiment, constructs and/or media samples were analysed by biochemical assay or RNA was extracted from constructs, reverse transcribed and gene expression quantified by RT-qPCR.
Parker et al. Arthritis Research & Therapy 2013 15:R163 doi:10.1186/ar4346