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B lymphocytes and B-cell activating factor promote collagen and profibrotic markers expression by dermal fibroblasts in systemic sclerosis

Antoine François1, Emmanuel Chatelus2, Dominique Wachsmann1, Jean Sibilia12, Seiamak Bahram1, Ghada Alsaleh1 and Jacques-Eric Gottenberg12*

Author Affiliations

1 Immunorhumatologie Moléculaire, INSERM UMR_S 1109, Centre de Recherche en Immunologie et Hématologie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, Strasbourg, France

2 Service de Rhumatologie, Centre National de Référence pour les Maladies Systémiques Autoimmunes Rares, Hôpitaux Universitaires de Strasbourg, Avenue Moliere, 67098 Strasbourg Cedex, France

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Arthritis Research & Therapy 2013, 15:R168  doi:10.1186/ar4352

Published: 28 October 2013

Additional files

Additional file 1: Figure S1:

Fibroblasts increase B-cell survival in vitro. B cells alone or cocultured with fibroblasts were seeded in 24-well plates for 3 or 5 days. For transwell experiments, B cells (5 × 105 cells) and HDF (105 cells) were seeded in the upper and lower chambers, respectively. After 3 or 5 days, B-cell viability was determined by FACS analysis; vital B cells were brightly positive when stained with DiOC6 and excluded PI. Data are expressed as the median of duplicate samples of two independent experiments ± interquartile range.

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