Phagocytosis of monosodium urate by osteoblasts. Confluent human OBs, previously stained with CellTracker Orange CMTMR, were cultured with vehicle (A) or with 0.5 mg MSU/106 cells (B) for 48 hours at 37°C, and then analyzed with confocal microscopy by using Olympus Fluoview microscope with helium-neon 543-nm lasers (left panel), differential interference contrast (DIC; middle panel), and overlap (right panel); ×400 magnification. Arrows indicate vacuoles and crystals of MSU. Representative of independent experiments with OB from three different donors.
Allaeys et al. Arthritis Research & Therapy 2013 15:R176 doi:10.1186/ar4365