IL-1β-stimulated degradation of FN1 and the production of its 60 kDa fragment is significantly reduced after carprofen + IL-1β treatment. (A) Representative FN western blot image. The band at about 230 kDa represents the glycosylated FN monomer. The 60 kDa band fragment was released when explants were stimulated with IL-1β. (B) Graphic representation of densitometry for FN. Explant cultures were completed for 6 days in three separate animals, with three treatment replicates on each occasion (n = 9). Explant treatments: untreated control, IL-1β (10 ng/ml), carprofen (100 μg/ml), or carprofen (100 μg/ml) + IL-1β (10 ng/ml). Error bars indicate standard deviation. An unpaired t-test was applied to assess significance. ***P < 0.001.
Williams et al. Arthritis Research & Therapy 2013 15:R223 doi:10.1186/ar4424