Additional file 2: Figure S2.

Cartilage explant cultures treated with IL-1β or carprofen + IL-1β did not show significant differences in β-actin release. (A) Representative western blot confirming the absence of β-actin in explant cultures that were untreated or treated with carprofen alone. IL-1β and carprofen + IL-1β treatments induced β-actin release, as evidenced by detection of an approximate 42 kDa band. (B) Graphic representation of the densitometric analysis of β-actin bands. An unpaired t-test was applied to assess statistical significance. ns, no significant difference; +, positive control for β-actin (equine chondrocyte lysate). Cartilage explants were cultured for 6 days with three treatment replicates from two animals (n = 6). Explant treatments: untreated control, IL-1β (10 ng/ml), carprofen (100 μg/ml), or carprofen (100 μg/ml) + IL-1β (10 ng/ml).

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Williams et al. Arthritis Research & Therapy 2013 15:R223   doi:10.1186/ar4424