Figure 1. Immunostaining for α-SMA in cutaneous samples. Representative tissue sample from: (A) Sham animal; (B) HOCl mice; (C) HOCl + PTU animal (original magnification, ×40). The arrows show strong diffuse staining of myofibroblast nuclei (dark brown staining). (D) Number of myofibroblasts from the three experimental groups (HOCl + PTU group, n = 10; HOCl group, n = 10; Sham, n = 5). The increase of myofibroblast population in the skin of HOCl mice is prevented by propylthiouracil administration. Values are expressed as the mean and standard deviation. *P <0.001 versus Sham; #P <0.001 versus HOCl. α-SMA, alpha-smooth muscle actin; HOCl, hypochlorous acid; HPF, high-powered field; PTU, propylthiouracil.
The authors declare that they have no competing interests.
Bagnato G, Bitto A, Irrera N, Pizzino G, Sangari D, Cinquegrani M, Roberts WN, Atteritano M, Altavilla D, Squadrito F, Bagnato G, Saitta A: Propylthiouracil prevents cutaneous and pulmonary fibrosis in the reactive oxygen species murine model of systemic sclerosis.