Expression of BAFF-R, CD40, CD80, and CD86 on peripheral B cells in IgG4-related disease (RD), primary Sjögren’s syndrome (pSS), and healthy controls (HC). Representative flow cytometry pictures of BAFF-R expression on CD19+ B cells of HC, IgG4-RD, and pSS patients (A). Scatter plots showing the percentages of CD19 + BAFF-R + cells in CD19+ cells (B). Scatter plots of the percentages of BAFF-R + expression on different B cell subsets (C-F). The changes in mean fluorescence intensity (MFI) of BAFF-R from healthy subjects after stimulation with recombinant human BAFF (rhBAFF) for 48 h (G). One representative example of decreased BAFF-R MFI on B cells by flow cytometry after 48 h culture in the presence of rhBAFF (2 ng/mL) (H). Representative flow cytometry of CD40 expression on CD19+ B cells of HC, IgG4-RD, and pSS patients (I). Scatter plots showing the percentages of CD19 + CD40+ cells in CD19+ cells (J-K). Percentages of CD80 and CD86 expression on CD19+ B cells of HC, IgG4-RD, and pSS patients (L,M). Values are shown as mean ± standard error of the mean, *P <0.05; **P <0.01; ***P <0.001.
Lin et al. Arthritis Research & Therapy 2014 16:R118 doi:10.1186/ar4571