Inhibitory effect of treatment with WAY-169916 on inflammatory gene expression in FLS induced by TNF-α. Fibroblast-like synoviocytes (FLS) were pretreated for 1 hour with WAY-169916 (1 μM) or pyrrolidine dithiocarbamate (PDTC) (100 μM) before treatment with tumor necrosis factor-α (TNF-α) for 1 hour. The mRNA levels for TNF-α, IL-6 and intercellular cell-adhesion molecule-1 (ICAM-1) were determined by real-time RT–PCR and normalized to glyceraldehyde-3-phosphate dehydrogenase. Results are reported as means ± SEM for each group, with the mean level of the stimulated cells treated with vehicle defined as 1. *P < 0.05 compared with vehicle control.
Keith et al. Arthritis Research & Therapy 2005 7:R427-R438 doi:10.1186/ar1692