CD134 is differentially expressed on CD4+ cells in secondary lymphoid organs during adjuvant arthritis. Popliteal lymph nodes (a,d), inguinal lymph nodes (b,e), and spleens (c,f) were isolated from Lewis rats before or during adjuvant arthritis (AA) development. Cell suspensions were stained for CD4 and CD134, and cell-associated fluorescence was analyzed by flow cytometry. Results for CD134 (black bars) are depicted as percentages of CD134+ cells of the CD4+ cell population and are expressed as means ± SEM (corrected for isotype control fluorescence). Results for CD4 (white bars) are shown as percentages of CD4+ cells of the live lymphocytes population and are expressed as means ± SEM (live lymphocytes gated based on forward scatter (FSC) and side scatter (SSC) profiles). The data shown are derived from four independent experiments and represent n = 5 to 7 rats per group for t = 0, n = 2 rats per group for t = 7, n = 3 to 7 rats per group for t = 10, n = 5 to 8 rats per group for t = 14, n = 5 to 9 rats per group for t = 21, and n = 4 to 6 rats per group for t = 35. *P < 0.05 compared with t = 0, **P < 0.01 compared with t = 0.
Boot et al. Arthritis Research & Therapy 2005 7:R604-R615 doi:10.1186/ar1722