Sequence of events in the murine air pouch model (a) Outline of a typical experiment. Air is injected subcutaneously on day 0 and repeated on day 3, as needed, to keep the pouch inflated. The root extracts or water are gavage-fed once daily on days 3–6. A suspension of MSU crystals in PBS (or PBS only) is injected into the pouch cavity on day 6 after the last gavage feeding. Pouch exudate and tissue are obtained for analysis 9 hours after crystal injection. (b) Determination of the time of maximal inflammation. The MSU crystal suspension was injected into the pouch at 0 hours. Leukocyte counts in the pouch exudate were determined by manual cell counting at the indicated time points (n = 4 mice for each time point). MSU, monosodium urate; PBS, phosphate-buffered saline.
Jung et al. Arthritis Research & Therapy 2007 9:R64 doi:10.1186/ar2222